Tommaso Castroflorio graduated in Dentistry and Dental Prosthetics at the University of Turin in 1998 and completed a Specialisation Course in Orthodontics (with honours and recommendation for publication of the thesis) at the same university in 2001. 

From 2001 to 2007 he was a subject expert and clinical instructor at the School of Specialisation in Orthodontics of the University of Turin.

He continued his university education by completing a PhD Course in Morphological Sciences at the University of Milan in 2007. 

In 2008 he attended an Advanced Training Course in Clinical Methodology of Headaches and Cranial Neuralgia at the University of Turin.

In 2009 he completed the Orofacial Pain Course of the School of Medicine and Dentistry at the University of New Jersey (USA).

He has been collaborating with the Laboratory for Engineering of the Neuromuscular System (LISIN) of the Polytechnic of Turin and with the Centre for Sensory-Motor Interaction of the University of Aalborg (Denmark) to develop research protocols focusing on the neuromuscular aspects of orofacial pain. He is currently collaborating with the Bernstein Center for Computational Neuroscience of the University of Gottingen (Germany) to study new protocols for the recording and processing of the electromyographic signals of the masticatory muscles.

Since 2007 he has been Visiting Professor of the Postgraduate Course in Interceptive Orthodontics at the University of Krems (Austria).

Since 2011 he has been working with the Department of Human Morphology of the University of Milan in order to define new quantitative investigation protocols in the fields of orthodontics and oral physiology.

Since 2012 he has been a clinical instructor at the School of Specialisation in Orthodontics of the University of Turin.

He teaches in master’s degree courses and advanced training courses offered by the most prestigious Italian universities on the topics of orthodontics and temporomandibular disorders.

In 2002 he received an award from the Italian Society of Orthodontics (Società Italiana di Ortodonzia - SIDO) for the best report presented on the topic of “Orthodontic finalization of dysfunctional patients”. 
He has also been awarded prizes and certificates of merit from the Italian Association of Dentistry Professors (Collegio dei Docenti di Odontoiatria) in 2003, 2004, and 2012 for his research projects in the fields of temporomandibular disorders and electrophysiology of masticatory muscles. He is the author of several publications in national and international journals and chapters of books.

He has presented his research results in Italy, the USA, the Netherlands, Spain, Denmark, Germany, and Iceland. 

He is a referee for the most prestigious international peer-reviewed journals on orthodontics and oral physiology. 

He was appointed Invisalign speaker for Europe in 2010 and he has been a key opinion-leader for Invisalign in Europe since 2011.

He is an ordinary member of the Italian Society of Orthodontics (SIDO), as well as a member of the Italian Association of Specialists in Orthodontics (ASIO) and of the European Orthodontic Society (EOS), a fellow of the World Federation of Orthodontists (WFO), and an international member of the American Association of Orthodontists (AAO).

Furthermore, he is a member of the Italian Society of Temporomandibular Pain (SIDA), of the International Association for the Study of Pain (IASP), and of the American Academy of Orofacial Pain (AAOP).

He is also a member of the Italian National Association of Dentists (Associazione Nazionale Dentisti Italiani - ANDI).